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Catalog Number | orb1933633 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB38026 |
Tested applications | IF, WB |
Predicted Reactivity | Mouse |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:1000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 49578 Da |
Target | This TFAP2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-336 amino acids from the C-terminal region of human TFAP2D. |
UniProt ID | Q7Z6R9 |
NCBI | NP_758438.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Transcription factor AP-2-delta, AP2-delta, Activa Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
TFAP2D Antibody (C-term) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the TFAP2D antibody detected the TFAP2D protein (arrow).
Fluorescent image of A549 cell stained with TFAP2D Antibody (C-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAP2D primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). TFAP2D immunoreactivity is localized to Nucleus significantly.