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Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:500-1:2000, IP: 1:20-1:100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1800-1900 of human TET1 (NP_085128.2). |
| Molecular Weight | 250kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TET1, CXXC6, LCX, bA119F7.1, MLL-TET1, TET1-MLL
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Immunofluorescence analysis of HeLa cells using TET1 Rabbit pAb (orb12705) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of paraffin-embedded mouse fetal Brain using TET1 Rabbit pAb (orb12705) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Western blot analysis of various lysates using TET1 Rabbit pAb (orb12705) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Quick Database Links
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Xiaojun Zhou 1 2, Shan Jiang 3 4, Siyi Guo 4, Shuai Yao 4, Qiqi Sheng 4, Qian Zhang 5, Jianjun Dong 4, Lin Liao C/EBPβ-Lin28a positive feedback loop triggered by C/EBPβ hypomethylation enhances the proliferation and migration of vascular smooth muscle cells in restenosis Chin Med J (Engl), (2024)
Applications
WB
Reactivity
Rat
TET1 Rabbit pAb (orb12705)
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