TET1 Antibody

• Catalog Number: orb1240105
• Category: Antibodies
• Description: TET1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-TET1 antibody (orb1240105) was raised against an 18 amino acid peptide near the carboxy terminus of human TET1. The immunogen is located within amino acids 2030 - 2080 of TET1.
• Concentration: 1 mg/mL
• Dilution range: WB: 1 μg/mL; IHC: 1 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot and Immunohistochemistry in human, mouse and rat samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 235 kDa Observed: 235 kDa
• Target: TET1
• UniProt ID: Q8NFU7
• NCBI: NP_085128
• Storage: TET1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: TET1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: TET1 Antibody: LCX, CXXC6, bA119F7.1, KIAA1676, LC
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

WB Validation of TET1 in Human Cell Lines. Loading: 15 µg of lysate Antibodies: TET1 orb1240105, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation of TET1 in Mouse Tissues. Loading: 15 µg of lysate Antibodies: TET1 orb1240105, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation of TET1 in Rat Tissues. Loading: 15 µg of lysate Antibodies: TET1 orb1240105, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of TET1 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Hela cells labeling TET1 with orb1240105 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).

Immunofluorescence Validation of TET1 in Mouse Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TET1 with orb1240105 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI antibody (blue).

Immunohistochemistry Validation of TET1 in Human Testis Tissue. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-TET1 antibody (orb1240105) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of TET1 in Mouse Kidney Tissue. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TET1 antibody (orb1240105) at 1 µg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of TET1 in Rat Liver Tissue. Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-TET1 antibody (orb1240105) at 1 µg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.