Tenofovir

SKU: orb1307900

Description

Tenofovir

Research Area

Infectious Disease & Virology, Pharmacology & Drug Discovery, Protein Biochemistry

Key Properties

• CAS Number: 147127-20-6
• MW: 287.21
• Purity: 99.92% (May vary between batches)
• Formula: C₉H₁₄N₅O₄P
• SMILES: C[C@H](CN1C=NC2=C1N=CN=C2N)OCP(O)(O)=O
• Target: HIV Protease,Reverse Transcriptase,HBV
• Solubility: Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:4 mg/mL (13.93 mM);H2O:2 mg/mL (6.96 mM)

Bioactivity

• In Vivo:
  4 μM Tenofovir completely inhibited the growth of HIVIIIB in MT-2 cells.Tenofovir inhibited hepatitis B virus (HBV) activity in HepG2 2.2.15, HepAD38 and HepAD79 cells. Tenofovir reduced the viral cytopathic effects of HIV-1(IIIB), HIV-2(ROD) and HIV(EHO) in MT-4 cells with EC50s of 1.15 μg/mL, 1.12 μg/mL, and 1.05 μg/mL.Tenofovir also reduced the viral cytopathic effects of SIV(mac251), SIV(B670), SHIV(89.6) and SHIV(89.6). Tenofovir also reduced the cytopathic effect of SIV(mac251),SIV(B670),SHIV(89.6) and SHIV(RTSHIV).Tenofovir showed unique activity against polynucleoside-resistant HIV expressing the Q151M mutation, and was less susceptible to viruses with the T69S insertion mutation.
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• In Vitro:
  4 μM Tenofovir completely inhibited the growth of HIVIIIB in MT-2 cells.Tenofovir inhibited hepatitis B virus (HBV) activity in HepG2 2.2.15, HepAD38 and HepAD79 cells. Tenofovir reduced the viral cytopathic effects of HIV-1(IIIB), HIV-2(ROD) and HIV(EHO) in MT-4 cells with EC50s of 1.15 μg/mL, 1.12 μg/mL, and 1.05 μg/mL.Tenofovir also reduced the viral cytopathic effects of SIV(mac251), SIV(B670), SHIV(89.6) and SHIV(89.6). Tenofovir also reduced the cytopathic effect of SIV(mac251),SIV(B670),SHIV(89.6) and SHIV(RTSHIV).Tenofovir showed unique activity against polynucleoside-resistant HIV expressing the Q151M mutation, and was less susceptible to viruses with the T69S insertion mutation.
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• Cell Research:
  Cells are plated into 48-well tissue culture plates (39,000 cells/mL) and allowed to grow for 48 h followed by treatment with vehicle or Tenofovir. Following the treatment period, cell viability is assessed using the MTT assay. The MTT assay relies on the conversion of tetrazolium dye 3-(4,5-dimethlthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to formazan by NAD(P)H-dependent oxidoreductases.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, HIVProtease, HIV Protease, HIV, HBV, Hepatitis B virus, Human immunodeficiency virus, GS1278, GS-1278, GS 1278, Tenofovir, ReverseTranscriptase, Reverse Transcriptase, TDF, PMPA

Compound Identifiers

PubChem CID

464205