Tenofovir Disoproxil

SKU: orb1300062

Description

Tenofovir Disoproxil (GS 4331) is a nucleotide analog reverse transcriptase inhibitor used in HIV and Hepatitis B research. It is widely applied in both in vitro antiviral assays and in vivo pharmacokinetic and efficacy studies, serving as a key experimental compound for investigating antiviral mechanisms and therapeutic strategies.

Research Area

Infectious Disease & Virology, Protein Biochemistry

Key Properties

• CAS Number: 201341-05-1
• MW: 519.44
• Purity: 99.17%
• Formula: C₁₉H₃₀N₅O₁₀P
• SMILES: C([C@H](OCP(OCOC(OC(C)C)=O)(OCOC(OC(C)C)=O)=O)C)N1C=2C(N=C1)=C(N)N=CN2
• Target: HIV Protease,HBV,Reverse Transcriptase
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (3.85 mM);DMSO:45 mg/mL (86.63 mM)

Bioactivity

• Cell Research:
  Human immortalized epithelial HK-2 cells were grown in a keratinocyte-free media with 50 μg/mL bovine pituitary extract and 5 ng/mL recombinant epithelial growth factor from Invitrogen. Cells were grown in a warm humidified incubator with constant settings of 37 °C and 5% CO2. HK-2 cells were plated into six-well tissue culture plates (750,000 cells/mL) and allowed to grow for 48 h. Media was replaced and cells were treated with a final concentration of 0, 1.5, 3.0, 4.75, 14.5, or 28.8 μM TFV for 24, 48, or 72 h. The vehicle was an equal volume of phosphate buffered saline (PBS). Abacavir was prepared in sterile water and cells were treated for 24 h with 0, 1.5, 3 or 6 μM of abacavir to evaluate renal sensitivity to an agent recognized to be less nephrotoxic. Following the treatment period, cells were collected with Trypsin-EDTA (0.25%) for sample analysis.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bis(POC)-PMPA, Human immunodeficiency virus, GS4331, GS-4331, GS 4331, Hepatitis B virus, HBV, HIVProtease, HIV, HIV Protease, inhibit, Inhibitor, Reverse Transcriptase, ReverseTranscriptase, Tenofovir Disoproxil

Compound Identifiers

PubChem CID

5481350