TEF-4 rabbit pAb

• Catalog Number: orb770642
• Category: Antibodies
• Description: Rabbit polyclonal antibody to TEF-4
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human TEAD2. AA range:71-120
• MW: 49kD
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-TEAD2 antibody, anti-TEF4 antibody, anti-Tran
• Note: For research use only

Immunofluorescence analysis of Hela cell. 1, TEF-4 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). β-actin Monoclonal Antibody (5B7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western Blot analysis of mouse-brain cells using TEF-4 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Immunofluorescence analysis of HepG2 cells, using TEAD2 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TEAD2 Antibody. The picture on the right is blocked with the synthesized peptide.