CCL25 Antibody (Biotin)

• Catalog Number: orb1272294
• Category: Antibodies
• Description: CCL25 Antibody (Biotin)
• Target: CCL25
• Clonality: Polyclonal
• Conjugation: Biotin
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Purification: Anti-hTECK specific antibody was purified by affinity chromatography and then biotinylated.
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTECK (Human TECK).
• UniProt ID: O15444
• Tested applications: ELISA, WB
• Application notes: ELISA:Sandwich:To detect hTECK by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human TECK (XP-5280) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTECK. Western Blot:To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TECK, Ckb15, SCYA25, TECK, C-C motif chemokine 25,
• Research Area: Immunology & Inflammation
• Note: For research use only
• NCBI: O15444
• Expiration Date: 12 months from date of receipt.

To detect hTECK by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human TECK (orb1272295) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hTECK.

To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.