TEC Antibody (Center)

SKU: orb1428525

Description

Rabbit polyclonal antibody to TEC.

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB03266
• Immunogen: Synthetic Peptide
• Target: TEC
• Molecular Weight: 73581

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

Tyrosine-protein kinase Tec, TEC, PSCTK4

Fluorescent image of Hela cell stained with TEC Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TEC primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). TEC immunoreactivity is localized to Cytoplasm significantly.

Fluorescent image of Hela cell stained with TEC Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TEC primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). TEC immunoreactivity is localized to Cytoplasm significantly.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

TEC Antibody (F190) western blot analysis in NCI-H292 cell line lysates (35 ug/lane). This demonstrates the TEC antibody detected the TEC protein (arrow).

Western blot analysis of anti-TEC Pab in mouse liver tissue lysate. TEC (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Quick Database Links

Gene Symbol

TEC

UniProt

P42680

RefSeq (Protein):NP_003206.2