TDP43 Rabbit mAb [KD Validated] Antibody

SKU: orb1950437

Description

DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.

Images & Validation

• Tested Applications: FC, ICC, IHC, WB
• Dilution Range: WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from TDP43
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ALS10; TAR DNA binding protein 43; TARDBP; TDP43;

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

Immunofluorescent analysis of Hela cells, using TDP43 Antibody.

Immunohistochemical analysis of paraffin-embedded human glioma, using TDP43 Antibody.

Western blot analysis of TDP43 expression in (1) HeLa cell lysate; (2) Mouse brain lysate; (3) Rat brain lysate.

Quick Database Links

UniProt

Q13148