TDP-43/TARDBP Antibody

SKU: orb1537941

Description

TDP-43/TARDBP Antibody

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution Range: ELISA, IHC, IHC-P (10 µg/ml), WB (1:1000 - 1:2000)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: K1B8
• Immunogen: Recombinant human TARDBP (1-260aa) purified from E. coli.
• Target: TDP-43 / TARDBP
• Purification: Protein G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TARDBP, ALS10, TAR DNA-binding protein-43, TDP-43, TAR DNA binding protein, TAR DNA-binding protein 43, TDP43

Cell lysates of HeLa (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TARDBP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Quick Database Links

Gene Symbol

TDP-43 / TARDBP