TCP1 alpha Antibody

SKU: orb151162

Description

Rat Anti-Mouse TCP1-alpha Monoclonal IgG2a

Images & Validation

• Tested Applications: FC, ICC, IF, IP, WB
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Bovine, Canine, Drosophila, Guinea pig, Hamster, Human, Monkey, Mouse, Other, Porcine, Rabbit, Rat, Saccharomyces, Yeast
• Application Notes:
  1 µg/ml of SMC-479 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

Key Properties

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 91a
• Immunogen: Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).
• Target: TCP1 alpha
• Molecular Weight: 60kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

p63, Tcp-1, TCP1 alpha, Ccta, TRic, Tp63, 21454, c-cpn, CCT, Tcp1, ccpn, AI528772, Cct1, Tcp 1, c cpn

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-TCP1-alpha Monoclonal Antibody, Clone 91a. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Centrosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TCP1-alpha Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of TCP1 alpha protein using Rat Anti-TCP1 alpha Monoclonal Antibody, Clone 91a. Primary Antibody: Rat Anti-TCP1 alpha Monoclonal Antibody at 1:1000.

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-TCP1-alpha Monoclonal Antibody, Clone 91a. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Centrosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-TCP1-alpha Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Quick Database Links

Gene Symbol

TCP1 alpha

UniProt

P11983

NCBI Gene ID

21454

RefSeq (Protein):NP_038714.2