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Item 1 of 3
TCP1 alpha Antibody: APC
Catalog Number: orb151172
| Catalog Number | orb151172 |
|---|---|
| Category | Antibodies |
| Description | Rat monoclonal antibody against TCP1 alpha conjugated to APC |
| Species/Host | Rat |
| Clonality | Monoclonal |
| Clone Number | 91a |
| Tested applications | ICC, IF, IHC |
| Reactivity | Bovine, Canine, Drosophila, Guinea pig, Hamster, Human, Monkey, Mouse, Other, Porcine, Rabbit, Rat, Saccharomyces, Yeast |
| Isotype | IgG2a |
| Immunogen | Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region). |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000), ICC/IF (1:100) |
| Conjugation | APC |
| MW | 60kDa |
| Target | TCP1 alpha |
| Entrez | 21454 |
| UniProt ID | P11983 |
| NCBI | NP_038714.2 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
| Alternative names | p63 antibody, Tcp-1 antibody, TCP1 alpha antibody, Read more... |
| Note | For research use only |
| Application notes | 1 µg/ml of SMC-479 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-TCP1-alpha Monoclonal Antibody, Clone 91a. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Centrosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TCP1-alpha Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of TCP1 alpha protein using Rat Anti-TCP1 alpha Monoclonal Antibody, Clone 91a. Primary Antibody: Rat Anti-TCP1 alpha Monoclonal Antibody at 1:1000.
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-TCP1-alpha Monoclonal Antibody, Clone 91a. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Centrosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-TCP1-alpha Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
