Tcfec Antibody

• Catalog Number: orb1246874
• Category: Antibodies
• Description: Tcfec Antibody
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-HITKQQTHPERN
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: Tcfec
• UniProt ID: Q9WTW4
• NCBI: NP_112475.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Tcfec, transcription factor EC , BB107417, MGC1074
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave one band at approx 37kDa and much stronger bands at approx 70+28kDa in Mouse Testis lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the stonger bands we observe given the calculated size of 35.1kDa according to Mouse NP_112475.1. All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).Immunohistochemistry:In paraffin embedded Mouse Thymus shows nuclear staining in sporadic cells of the medula. Recommended concentration: 4-6ug/ml. Paraffin embedded Human Kidney and Spleen. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246874 (4 ug/ml) staining of paraffin embedded Mouse Thymus. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1246874 Negative Control showing staining of paraffin embedded Mouse Thymus, with no primary antibody.

orb1246874 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246874 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.