TCF4 Antibody: Biotin

• Catalog Number: orb413794
• Category: Antibodies
• Description: Rabbit polyclonal antibody against TCF4 conjugated to Biotin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse
• Immunogen: Synthetic peptide from the C-terminal of Human TCF4 (500-600 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); IHC (1:50)
• Conjugation: Biotin
• MW: 71 kDa, 45 kDa
• Target: TCF4
• Entrez: 6925
• UniProt ID: P15884
• NCBI: NP_001077431.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: TCF-4 antibody, MGC149723 antibody, PTHS antibody,
• Note: For research use only
• Application notes: A 1:1000 dilution of SPC-723 was sufficient for detection of TCF4 in 15 µg of mouse liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Rabbit Anti-TCF4 Polyclonal Antibody. Tissue: Kidney. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-TCF4 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Western blot analysis of Mouse liver lysate showing detection of ~71.3 kDa TCF4 protein using Rabbit Anti-TCF4 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse liver lysate. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF4 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71.3 kDa. Other Band (s): ~45 kDa isoform.