TCF3 Antibody (C-term)

• Catalog Number: orb1936235
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This TCF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506-534 amino acids from the C-terminal region of human TCF3.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P15923
• MW: 67600 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB33747
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Transcription factor E2-alpha, Class B basic helix
• Note: For research use only
• NCBI: NP_003191.1, NP_001129611.1

TCF3 Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35 ug/lane). This demonstrates the TCF3 antibody detected the TCF3 protein (arrow).

TCF3 Antibody (C-term) western blot analysis in human uterine tumor tissue lysates (35 ug/lane). This demonstrates the TCF3 antibody detected the TCF3 protein (arrow).

TCF3 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-TCF3 Antibody (C-term) at 1:1000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: Ramos whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

TCF3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TCF3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.