TCF3 Antibody (C-term)

• Catalog Number: orb1936235
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB33747
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 67600 Da
• Target: This TCF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506-534 amino acids from the C-terminal region of human TCF3.
• UniProt ID: P15923
• NCBI: NP_003191.1, NP_001129611.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Transcription factor E2-alpha, Class B basic helix
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

TCF3 Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35 ug/lane). This demonstrates the TCF3 antibody detected the TCF3 protein (arrow).

TCF3 Antibody (C-term) western blot analysis in human uterine tumor tissue lysates (35 ug/lane). This demonstrates the TCF3 antibody detected the TCF3 protein (arrow).

TCF3 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-TCF3 Antibody (C-term) at 1:1000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: Ramos whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

TCF3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TCF3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.