TBX5 Antibody (N-term)

• Catalog Number: orb1935579
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB34604
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:2000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 57711 Da
• Target: This TBX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TBX5.
• UniProt ID: Q99593
• NCBI: NP_000183.2, NP_852259.1, NP_542448.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: T-box transcription factor TBX5, T-box protein 5,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

TBX5 Antibody (N-term) western blot analysis in CEM cell line lysates (35 ug/lane). This demonstrates the TBX5 antibody detected the TBX5 protein (arrow).

TBX5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TBX5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-TBX5 Antibody (N-term) at 1:2000 dilution. Lane 1: Hela whole cell lysates. Lane 2: HepG2 whole cell lysates. Lane 3: HL-60 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.