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TBX5 Antibody (N-term)
Description
Research Area
Images & Validation
−| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:1000, IHC-P - 1:100-500 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 57711 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |

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TBX5 Antibody (N-term) western blot analysis in CEM cell line lysates (35 ug/lane). This demonstrates the TBX5 antibody detected the TBX5 protein (arrow).

TBX5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TBX5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-TBX5 Antibody (N-term) at 1:2000 dilution. Lane 1: Hela whole cell lysates. Lane 2: HepG2 whole cell lysates. Lane 3: HL-60 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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Protocol Information
TBX5 Antibody (N-term) (orb1935579)
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