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Catalog Number | orb1535058 |
---|---|
Category | Antibodies |
Description | TBX21/T-bet Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | A 19 amino acid peptide near the C-Terminus of human TBX2. The immunogen is located within the last 50 amino acids of TBX21 . |
Concentration | 1 mg/ml |
Dilution range | ELISA, IF (5 µg/ml), IHC, IHC-P (10 µg/ml), WB (1 µg/ml) |
Conjugation | Unconjugated |
Target | TBX21 / T-bet |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide |
Alternative names | TBX21, T-bet, TBET, T-box 21, T-box protein 21, TB Read more... |
Note | For research use only |
Application notes | Further information: TBX21 antibody can be used for detection of TBX21 by Western blot at 1 - 2 ug/ml. Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 59 kDa Observed: 55kDa |
Expiration Date | 12 months from date of receipt. |
Anti-TBX21 / T-bet antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Immunofluorescence of TBX21 in 293 cells with TBX21 antibody at 5 ug/ml.
Western blot analysis of TBX21 in 293 cell lysate with TBX21 antibody at 1 ug/ml.