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Catalog Number | orb1937892 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB19886 |
Tested applications | IF, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:500, IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 46554 Da |
Target | This TBC1D13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-105 amino acids from the Central region of human TBC1D13. |
UniProt ID | Q9NVG8 |
NCBI | NP_060671.3 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | TBC1 domain family member 13, TBC1D13 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-TBC1D13 Antibody (Center) at 1:500 dilution. Lane 1:PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 47kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of TBC1D13 Antibody (Center) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
TBC1D13 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TBC1D13 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
TBC1D13 Antibody (Center) western blot analysis in CEM cell line lysates (35 ug/lane). This demonstrates the TBC1D13 antibody detected the TBC1D13 protein (arrow).
TBC1D13 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35 ug/lane). This demonstrates the TBC1D13 antibody detected the TBC1D13 protein (arrow).