TBB5 Antibody

• Catalog Number: orb1939448
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 87CT59.3.7
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Hamster, Other, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2b,Igκ
• Dilution range: IF: 1:25, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:10~50
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 49671 Da
• Target: TBB5 recombinant protein is used to produce this monoclonal antibody.
• UniProt ID: P07437
• NCBI: NP_821133.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Tubulin beta chain, Tubulin beta-5 chain, TUBB, TU
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human testis reacted with TBB5 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescent analysis of A549 cells, using TBB5 Antibody. diluted at 1：25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded H.kidney section using TBB5 Antibody. diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from 293, CEM, Hela, Y79, rat PC-12 cell line, mouse brain tissue lysate (from left to right), using TBB5 Antibody. diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary Antibody. Lysates at 20 μg per lane.