TBB1 Antibody

SKU: orb1939452

Description

TBB1 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: WB - 1:500-1000, IHC-P - 1:100-500, IF - 1:10-50
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Molecular Weight: 50327 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

All lanes: Anti-TBB1 Antibody at 1:1000 dilution. Lane 1: CCRF-CEM whole cell lysate. Lane 2: Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of TBB1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human brain reacted with TBB1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-TBB1 Antibody in mouse brain tissue, Y79, CEM and 293 lysates (35 μg/lane). TBB1 (arrow) was detected using the purified Mab. (1 μg/ml)

Quick Database Links

UniProt

Q9H4B7