TBB1 Antibody

• Catalog Number: orb1939452
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 86CT4.4.4
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:500~1000, IHC-P: 1:10~50
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 50327 Da
• Target: This monoclonal antibody is generated from mice immunized with a recombinant protein human TBB1.
• UniProt ID: Q9H4B7
• NCBI: NP_110400.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Tubulin beta-1 chain, TUBB1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-TBB1 Antibody at 1:1000 dilution. Lane 1: CCRF-CEM whole cell lysate. Lane 2: Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of TBB1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human brain reacted with TBB1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-TBB1 Antibody in mouse brain tissue, Y79, CEM and 293 lysates (35 μg/lane). TBB1 (arrow) was detected using the purified Mab. (1 μg/ml)