TAU (MAPT) Antibody (S720)

SKU: orb1935978

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:10-50, IHC - 1:25
• Reactivity: Human, Monkey
• Predicted Reactivity: Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This TAU(MAPT) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 699-728 amino acids from human TAU(MAPT). Antigen Region: 699-728 aa.
• Target: MAPT (HGNC:6893)
• Molecular Weight: 78928 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPT, MAPTL, MTBT1, TAU

MAPT Antibody (pS720) western blot analysis in SH-SY5Y cell line lysates (35 ug/lane). This demonstrates the MAPT antibody detected the MAPT protein (arrow).

Formalin-fixed and paraffin-embedded human brain tissue reacted with MAPT Antibody (S720), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Staining TAU in Monkey brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

MAPT (HGNC:6893)

UniProt

P10636

RefSeq (Protein):NP_001116539.1, NP_005901.2, NP_058519.3, NP_058525.1, NP_001190181.1, NP_001116538.2, NP_058518.1