TAU Antibody (C-term)

SKU: orb1931768

Description

TAU Antibody (C-term)

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: IHC-P - 1:100-500, WB - 1:1000, IF - 1:10-50
• Reactivity: Human, Monkey, Mouse, Rat
• Predicted Reactivity: Bovine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 78928 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MAPTL, MTBT1, TAU

Confocal immunofluorescent analysis of TAU Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Staining TAU in Cynomolgus Monkey brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Western blot analysis of TAU (arrow) using rabbit polyclonal TAU Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TAU gene (Lane 2).

All lanes: Anti-TAU Antibody (K692) at 1:2000 dilution. Lane 1: rat brain lysate. Lane 2: mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 79 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P10636