TAU Antibody (C-term)

SKU: orb1931768

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:2000, IHC-P - 1:25
• Reactivity: Human, Monkey, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This TAU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 677-707 amino acids from the C-terminal region of human TAU. Antigen Region: 677-707 aa.
• Target: MAPT (HGNC:6893)
• Molecular Weight: 78928 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPT, MAPTL, MTBT1, TAU

Confocal immunofluorescent analysis of TAU Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Staining TAU in Cynomolgus Monkey brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Western blot analysis of TAU (arrow) using rabbit polyclonal TAU Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TAU gene (Lane 2).

All lanes: Anti-TAU Antibody (K692) at 1:2000 dilution. Lane 1: rat brain lysate. Lane 2: mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 79 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

MAPT (HGNC:6893)

UniProt

P10636

RefSeq (Protein):NP_001116539.1, NP_005901.2, NP_058519.3, NP_058525.1, NP_001190181.1, NP_001116538.2, NP_058518.1