Stargazin Antibody (APC)

SKU: orb150200

Description

Mouse monoclonal to TARP Gamma2 (APC). Stargazin belongs to a group called voltage gated calcium channels (VGCCs) which are present in most excitable cells. Stargazin is thought to stabilize the calcium channel in an inactivated (closed) state. There are five high voltage activated calcium channel types (L, N, P, Q, and R) and one low voltage activated channel type (T). Each of these channels exists as a heteromultimer of a1, b, a2/ d and g subunits with the voltage activated calcium channel function carried by the a subunits. VGCCs exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival..

Research Area

Disease Biomarkers, Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-422 was sufficient for detection of TARP Gamma2/4/8 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2A
• Clone No.: N245/36 (Formerly sold as S245-36)
• Immunogen: Fusion protein amino acids 203-323 (Cytoplasmic C-terminus) of rat TARPGamma2
• Target: Stargazin
• Molecular Weight: 35-55kDa
• Purification: Protein G Purified
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CACNG2, Calcium channel voltage dependent gamma subunit 2, CaV gamma 2, Ipr328, MGC123981, MGC138502, MGC138504, Voltage-dependent calcium channel gamma-2 subunit, Neuronal voltage-gated calcium channel gamma-2 subunit, Stargazin, Stg, Stargazer, TARP, TARP gamma-2, Transmembrane AMPAR regulatory protein gamma-2, AW060990, B230105C07Rik, B930041E13Rik, Wag, Waggler

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone N245/36. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody (D) Composite.

Western blot of rat brain lysate showing detection of ~55 kDa TARP Gamma2-4-8 protein using Anti-Tarp Gamma2-4-8 antibody [N245/36]. ?Lane 1: MW ladder. Lane 2: Anti-TARP Gamma2-4-8 (1:100). Lane 3: Anti-TARP Gamma2-4-8 (1:250). Lane 4: Anti-TARP Gamma2-4-8 (1:500). Lane 5: Anti-TARP Gamma2-4-8 (1:1000). Load: 20 μg RB lysate per lane. Block: 5% milk + TBST 1hr at RT. Primary antibody: Mouse Anti-TARP Gamma2-4-8 primary antibody [245-36] incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody at 1:50 for 60 min at RT. Colour Development: TMB solution for 30 min at RT. Predicted/Observed size: ~ 55 kDa. Other band (s) (faint): ~37kDa, ~40kDa, ~75kDa.

Quick Database Links

Gene Symbol

Stargazin

UniProt

Q71RJ2

NCBI Gene ID

84347

RefSeq (Protein):NP_445803.1