TARDBP Antibody

• Catalog Number: orb1261135
• Category: Antibodies
• Description: TARDBP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human TARDBP (NP_031401.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:20 - 1:100IP: 1:50 - 1:100RIP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 42kDa
• Target: TARDBP
• UniProt ID: Q13148
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: TDP43 Antibody: ALS10, TDP-43, TDP43, TAR DNA-bind
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:20 - 1:100IP: 1:50 - 1:100RIP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using TARDBP antibody (orb1261135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded human esophagus using TARDBP Antibody (orb1261135) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human endometrium using TARDBP Antibody (orb1261135) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using TARDBP antibody (orb1261135). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of SW620 cells using 3 ug TARDBP antibody (orb1261135). Western blot was performed from the immunoprecipitate using TARDBP antibody (orb1261135) at a dilution of 1:500.