TARDBP Antibody

SKU: orb1246608

Description

TARDBP Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 45kDa band observed in lysates of cell lines Jurkat (calculated MW of 44.7kDa according to NP_031401.1). A slightly larger protein is detected in some regions of the Brain. Recommended concentration: 0.3-1µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-HISNAEPKHNSNRQ
• Target: TARDBP
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TARDBP, TAR DNA binding protein, ALS10, TDP-43, OTTHUMP00000002171, TAR DNA-binding protein-43

orb1246608 (0.3 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1246608 (1 ug/ml) staining of Human Cerebellum (A), Frontal Cotex (B) and Hippocampus (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

TARDBP

UniProt

Q13148

RefSeq (Protein):NP_031401.1