Talin-1 rabbit pAb Antibody

SKU: orb767181

Description

Rabbit polyclonal antibody to Talin-1

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from the C-terminal region of human TLN1. AA range:2471-2520
• Molecular Weight: 269
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-TLN1 antibody, anti-KIAA1027 antibody, anti-TLN antibody, anti-Talin-1 antibody

Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Western Blot analysis of K562 cells using Talin-1 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000.

Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100.

Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100.