TAL1 Antibody (T90)

SKU: orb1931484

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P-Leica - 1:500
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB15312
• Immunogen: This TAL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from human TAL1. Antigen Region: 68-97 aa.
• Target: TAL1
• Molecular Weight: 34271 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

T-cell acute lymphocytic leukemia protein 1, TAL-1, Class A basic helix-loop-helix protein 17, bHLHa17, Stem cell protein, T-cell leukemia/lymphoma protein 5, TAL1, BHLHA17, SCL, TCL5

Immunohistochemical analysis of paraffin-embedded Human marrow tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-TAL1 Antibody (T90) at 1:1000 dilution. Lane 1: CCRF-CEM whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: MOLT-4 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 34 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

TAL1

UniProt

P17542

RefSeq (Protein):NP_003180.1