TAGLN/Transgelin/SM22 Antibody

• Catalog Number: orb1537823
• Category: Antibodies
• Description: TAGLN/Transgelin/SM22 Antibody
• Target: TAGLN / Transgelin / SM22
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Equine, Hamster, Human, Mammal, Monkey, Mouse, Porcine, Rat, Xenopus
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence C-MTGYGRPRQIIS, from the C-Terminus of protein sequence according to NP_003177.2.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (2.5 - 5 µg/ml), Peptide-ELISA (1:8000), WB (0.01 - 0.03 µg/ml)
• Application notes: Further information: Immunohistochemistry: LS-B2563 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2563 was determined to be 5 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TAGLN, 22 kDa actin-binding protein, SMCC, TAGLN1,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

Antibody staining (0.001 ug/ml) of Human Placenta lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.