TAG-72 Antibody [CC49]

• Catalog Number: orb1252369
• Category: Antibodies
• Description: TAG-72 Antibody [CC49]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CC49
• Tested applications: FC, IF, IHC, WB
• Reactivity: Bovine, Canine, Human, Rat
• Isotype: IgG1
• Immunogen: Purified TAG-72 protein was used as the immunogen for this TAG-72 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the TAG-72 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• UniProt ID: NotKnown
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the TAG-72 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with TAG-72 antibody (B72.3 + CC49).

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with TAG-72 antibody (B72.3 + CC49).

Western blot testing of Jurkat cell lysate with TAG-72 antibody (clone CC49). Expected molecular weight ~220kDa.