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Catalog Number | orb1252647 |
---|---|
Category | Antibodies |
Description | TAG-72 Antibody [CA72/733] |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | CA72/733 |
Tested applications | FC, IF, IHC |
Reactivity | Bovine, Canine, Human, Rat |
Isotype | IgG1, kappa |
Immunogen | Recombinant human TAG-72 protein was used as the immunogen for this antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TAG-72 antibody to be titered up or down for optimal performance.1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TAG-72 antibody to be titered up or down for optimal performance.1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of human prostate carcinoma with TAG-72 antibody (CA72/733).
IHC testing of human ovarian carcinoma with TAG-72 antibody.
IHC staining of human colon cancer with TAG-72 antibody.
Bovine, Canine, Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
Bovine, Canine, Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
Bovine, Canine, Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
Bovine, Canine, Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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