TADA3L Antibody (C-term)

• Catalog Number: orb1935508
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB35022
• Tested applications: IF, WB
• Predicted Reactivity: Mouse, Other, Rat, Zebrafish
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 48902 Da
• Target: This TADA3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 386-414 amino acids from the C-terminal region of human TADA3L.
• UniProt ID: O75528
• NCBI: NP_006345.1, NP_001265199.1, NP_597814.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Transcriptional adapter 3, ADA3 homolog, hADA3, ST
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

TADA3L Antibody (C-term) western blot analysis in Hela cell line lysates (35 ug/lane). This demonstrates the TADA3L antibody detected the TADA3L protein (arrow).

Fluorescent confocal image of U251 cell stained with TADA3L Antibody (C-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TADA3L primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TADA3L immunoreactivity is localized to Nucleus significantly.