TA-01

SKU: orb1302686

Description

TA-01 is a highly potent small molecule inhibitor of CK1ε, CK1δ, and p38 MAPK, exhibiting IC50 values in the low nanomolar range. This dual-activity profile makes it a valuable research tool for investigating circadian rhythms, inflammation, and neurodegenerative diseases in both cellular and animal models.

Research Area

Cell Biology, Metabolism Research, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 1784751-18-3
• MW: 351.32
• Purity: 99.94% (May vary between batches)
• Formula: C₂₀H₁₂F₃N₃
• SMILES: Fc1ccc(cc1)-c1nc([nH]c1-c1ccncc1)-c1c(F)cccc1F
• Target: Autophagy,Casein Kinase,p38 MAPK
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (2.85 mM);DMSO:9 mg/mL (25.62 mM)

Bioactivity

• Target IC50:
  CKIϵ:6.4 nM |p38 MAPk:6.7 nM|CKIδ:6.8 nM
• In Vitro:
  TA-01, a potent inhibitor of CK1 and p38 MAPK, demonstrates IC50 values of 6.4 nM for CK1ε, 6.8 nM for CK1δ, and 6.7 nM for p38 MAPK. At a concentration of 5 µM, TA-01 is non-cytotoxic and completely inhibits cardiogenesis, although it induces cardiogenesis at lower concentrations.
• Cell Research:
  HES-3, H7 and IPS are harvested and seeded at 1.1 × 106 cells/mL as EBs in ultra-low attachment 12-well plates in bSFS medium: DMEM supplemented with 2 mM l-glutamine, 0.182 mM sodium pyruvate, 1% non-essential amino acids, 0.1 mM β-mercaptoethanol, 5.6 mg/L transferrin, 20 μg/L sodium selenite, 0.25% (w/vol) Bovine Serum Albumin and 0.25% (w/vol) Hysoy. Cells are incubated at 37°C and 5% CO2 to allow EB formation. The medium is refreshed after 1 day and then every 2-3 days. Cells are stimulated with the respective compounds (TA-01) dissolved in DMSO (1 μL DMSO/mL of media) starting from day 1 or day 4, until day 8. CHIR99021 is applied for the first 24 h only.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, CaseinKinase, Casein Kinase, Autophagy, CK1δ, CK1ϵ, TA 01, TA01, TA-01, p38 MAPK, p38MAPK

Compound Identifiers

PubChem CID

91691129