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Catalog Number | orb1787854 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Target | This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide of T7 tag. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
UniProt ID | NA |
MW | 45-50KD |
Tested applications | WB |
Dilution range | WB: 1:1000 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Note | For research use only |
All lanes: Anti-T7 tag Antibody at 1:1000 dilution. Lane 1: 293T/17 transfected with 12tag lysate (1ug). Lane 2: Non-transfected 293T/17 lysate (1ug). Lane 3: 12tag recombinant protein lysate (0. 1ug). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
WB | |
All | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |