T56-LIMKi

SKU: orb1303512

Description

T56-LIMKi (T5601640) is a potent and selective small molecule inhibitor targeting LIMK2. This compound is a valuable research tool for investigating LIMK2's role in cytoskeletal dynamics, cell motility, and metastasis in both cellular and animal models of cancer and neurological disorders.

Research Area

Cell Biology

Key Properties

• CAS Number: 924473-59-6
• MW: 389.33
• Purity: 99.57% (May vary between batches)
• Formula: C₁₉H₁₄F₃N₃O₃
• SMILES: Cc1cc(on1)C(=O)Nc1cccc(c1)C(=O)Nc1cccc(c1)C(F)(F)F
• Target: LIM Kinase
• Solubility: 10% DMSO+90% Corn Oil:2.5 mg/mL (6.42 mM);DMSO:50 mg/mL (128.43 mM)

Bioactivity

• Target IC50:
  LIMK2:35.2 μM.
• In Vivo:
  T56-LIMKi induces inhibition of cofilin phosphorylation and promotes Panc-1 tumor shrinkage in vivo, with mice treated with T56-LIMKi (60 mg/kg) showing a significant decrease in tumor volume compared to controls.
• In Vitro:
  T56-LIMKi effectively suppresses the proliferation of various cancer cell lines, including ST88-14, U87, Panc-1, and A549 lung cancer cells, exhibiting IC50 values of 18.3, 7.4, 35.2, and 90 µM respectively. It significantly lowers phosphorylated cofilin (p-cofilin) levels, impairing the growth of pancreatic, glioma, and schwannoma cancer cells. This compound inhibits cofilin phosphorylation, crucial for actin disruption, thereby preventing tumor cell movement, proliferation, and the formation of colonies in soft agar without contact. At concentrations ranging from 10-50 µM, T56-LIMKi dose-dependently decreases p-cofilin in NF1−/− MEFs, with a 30 µM IC50, while not affecting total cofilin levels. Furthermore, a 50 µM dosage of T56-LIMKi significantly reduces the presence of stress fibers in cells.
• Cell Research:
  T56-LIMKi can induce inhibition of cofilin phosphorylation and Panc-1 tumor shrinkage in vivo. Mice treated with T56-LIMKi (60 mg/kg) shows a significant decrease in tumor volume compared to control.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, LIM Kinase, LIM Kinase (LIMK), LIMK2, LIMKinase, LIMKs, Inhibitor, T 5601640, T56 LIMKi, T5601640, T-5601640, T56LIMKi, T-56-LIMKi, T56-LIMKi

Compound Identifiers

PubChem CID

9438169