SYVN1 (HRD1) Antibody (C-term)

• Catalog Number: orb1939521
• Category: Antibodies
• Description: SYVN1 (HRD1) Antibody (C-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q86TM6
• MW: 67685 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: WB - 1:1000, IF - 1:200, IHC-P - 1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: HRD1, KIAA1810
• Research Area: Cell Biology, Protein Biochemistry, Signal Transdu
• Note: For research use only

All lanes: Anti-HRD1 Antibody (A601) at 1:2000 dilution. Lane 1: DU145 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: mouse spleen lysate. Lane 4: PC-3 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-HRD1 Antibody (A601) at 1:2000 dilution + MOLT-4 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of HeLa cells stained with SYVN1 (HRD1) (C-term) Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with SYVN1 (HRD1) (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human Liver tissue reacted with SYVN1 (HRD1) Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Mouse Neuroblastoma Neuro2A (N2A) was transiently transfected, collected at 72h after transfection. Primary antibodies against syvn1 and anti-rabbit secondary POD-conjugated antibodies.