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Syntaxin 1 (phospho Ser14) rabbit pAb

Catalog Number: orb770189

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb770189
CategoryAntibodies
DescriptionRabbit polyclonal antibody to Syntaxin 1 (phospho-Ser14)
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC-P, WB
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human Syntaxin 1A around the phosphorylation site of Ser14. AA range:1-50
Concentration1 mg/ml
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
ConjugationUnconjugated
MW35
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Alternative namesAnti-STX1A antibody, anti-STX1 antibody, anti-Synt
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NoteFor research use only
Expiration Date12 months from date of receipt.
Syntaxin 1 (phospho Ser14) rabbit pAb

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Syntaxin 1 (phospho Ser14) rabbit pAb

Immunofluorescence analysis of NIH/3T3 cells, using Syntaxin 1A (Phospho-Ser14) Antibody. The picture on the right is blocked with the phospho peptide.

Syntaxin 1 (phospho Ser14) rabbit pAb

Western blot analysis of lysates from 293 cells, using Syntaxin 1A (Phospho-Ser14) Antibody. The lane on the right is blocked with the phospho peptide.