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Catalog Number | orb1538549 |
---|---|
Category | Antibodies |
Description | SYNPO/Synaptopodin Antibody |
Target | SYNPO / Synaptopodin |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide |
Purification | Peptide Column Affinity Chromatography |
Immunogen | A 17 AA synthetic peptide near the C-Terminus of human SYNPO. The immunogen is located within the last 50 AA of SYNPO. |
Tested applications | ELISA, IF, IHC, IHC-P, WB |
Dilution range | ELISA, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml) |
Application notes | Further information: SYNPO antibody can be used for detection of SYNPO by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 75, 99, 102 kDa Observed: 95 kDa |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | SYNPO, KIAA1029, Synaptopodin |
Note | For research use only |
Immunofluorescence of SYNPO in human brain tissue with SYNPO antibody at 20 ug/ml.
Western blot analysis of SYNPO in rat brain tissue lysate with SYNPO antibody at (A) 1 and (B) 2 ug/ml.