Synaptophysin Antibody

• Catalog Number: orb1822628
• Category: Antibodies
• Description: This recombinant mouse monoclonal antibody recognizes a protein of 38kDa that is identified as synaptophysin. It is an N-glycosylated integral membrane protein found in neurons and endocrine cells. Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel.This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation.
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant partial protein sequence (within amino acids 224-313) from the human protein was used as the immunogen for the SYP antibody.
• UniProt ID: P08247
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Antibody Type: Primary Antibody
• Clone Number: rSYP/8807
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Aliquot the SYP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Hazard Information: This SYP antibody is available for research use only.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human pancreas tissue with SYP antibody (clone rSYP/8807). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free SYP antibody (clone rSYP/8807) as confirmation of integrity and purity.