Symmetric DiMethyl-Histone H3-R17 pAb

• Catalog Number: orb1257937
• Category: Antibodies
• Description: Symmetric DiMethyl-Histone H3-R17 pAb
• Target: H3R17me2s
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding Arg17 of human histone H3
• UniProt ID: P68431
• MW: Observed: 17kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H3/b, H3FB, ERBB, ERBB1, HER1, H3/a, H3/c, H3/d, H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H3-R17 antibody (orb1257937). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R17 antibody (orb1257937).

Immunohistochemistry of paraffin-embedded human stomach using H3R17me2s antibody (orb1257937) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R17 antibody (orb1257937). Blue: DAPI for nuclear staining.