SYCE1 Antibody

SKU: orb1256523

Description

SYCE1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 132-351 of human SYCE1 (NP_001137236.1).
• Target: SYCE1
• Molecular Weight: Observed: 40kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

C10orf94, CT76, POF12, SPGF15, synaptonemal complex central element protein 1, cancer/testis antigen 76, epididymis secretory sperm binding protein

Western blot analysis of extracts of various cell lines, using SYCE1 antibody (orb1256523) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of MCF-7 cells using SYCE1 antibody (orb1256523). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

SYCE1

UniProt

Q8N0S2