Swine IgG (H&L) Antibody Alkaline Phosphatase Conjugated

• Catalog Number: orb347982
• Category: Antibodies
• Description: Swine IgG (H&L) antibody (Alkaline Phosphatase)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: AP
• Reactivity: Porcine
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Swine IgG, and Swine Serum.
• Immunogen: Swine IgG whole molecule
• Tested applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:14,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application notes: Anti-Swine IgG Alkaline Phosphatase Conjugated antibody has been tested by ELISA, dot blot, and western blot and is suitable for use in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Alternative names: Rabbit Anti-Swine IgG alkaline phosphatase Conjuga
• Note: For research use only

Western Blot of Alkaline Phosphatase conjugated Rabbit anti-Swine IgG antibody. Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase swine secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min RT. Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG. Other band(s): none.