F(ab')2 Swine IgG (H&L) antibody (Alkaline Phosphatase)

• Catalog Number: orb348398
• Category: Antibodies
• Description: F(ab')2 Swine IgG (H&L) antibody (Alkaline Phosphatase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Porcine
• Isotype: IgG F(ab')2
• Immunogen: Swine IgG whole molecule
• Concentration: 0.5 mg/mL
• Dilution range: ELISA: 1:8,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Alkaline Phosphatase, Swine IgG and Swine Serum. No reaction was observed against anti-Pepsin and anti-Rabbit IgG F(c).
• Conjugation: AP
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rabbit F(ab')2 Anti-Swine IgG Antibody Alkaline Ph
• Note: For research use only
• Application notes: This product has been assayed against 1.0 µg of Swine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.