Swine IgG (H&L) Antibody Alkaline Phosphatase Conjugated

SKU: orb347967

Description

Swine IgG (H&L) antibody (Alkaline Phosphatase)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Reactivity: Porcine
• Application Notes:
  Anti-Swine IgG Alkaline Phosphatase Conjugated Antibody has been tested by dot blot and ELISA and is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other antibody based enzymatic assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Swine IgG whole molecule was produced by repeated immunization with Swine IgG whole molecule in goat.
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Swine IgG and Swine Serum.
• Conjugation: AP

Storage & Handling

• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat Anti-Swine IgG alkaline phosphatase Conjugated Antibody, goat Anti-Swine IgG Antibody alk phos Conjugation

Dot Blot of Goat anti-Swine IgG Antibody Alkaline Phosphatase Conjugated. Antigen: Swine IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Swine IgG Antibody Alkaline Phosphatase Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 1 HR at RT.