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Swine Human C3c, conjugated with TRITC Antibody

SKU: orb20829

Description

Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal swine antiserum to C3c fragment of human complement factor C3. C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. C3c is isolated and purified from pooled normal human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Research Area

Epigenetics, Immunology

Images & Validation

ReactivityHuman

Key Properties

Antibody TypePrimary Antibody
HostPorcine
ClonalityPolyclonal
ConjugationTRITC

Storage & Handling

StorageThe lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Form/AppearanceFluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.
DisclaimerFor research use only
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Protocol Information

Swine Human C3c, conjugated with TRITC Antibody (orb20829)

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1 ml
$ 490.00
DispatchUsually dispatched within 5-10 working days
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