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SUZ12 Antibody
SKU: orb1274421
Featured
Description
Images & Validation
−Item 1 of 10
| Tested Applications | IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 470-739 of human SUZ12 (NP_056170.2). |
| Target | SUZ12 |
| Molecular Weight | Observed: 83kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−SUZ12 Antibody: CHET9, JJAZ1, CHET9, KIAA0160, Polycomb protein SUZ12, Chromatin precipitated E2F target 9 protein, ChET 9 protein
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Western blot analysis of extracts of various cell lines, using SUZ12 Antibody (orb1274421) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and SUZ12 knockout (KO) HeLa cells, using SUZ12 antibody (orb1274421) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using SUZ12 antibody (orb1274421) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using SUZ12 antibody (orb1274421) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using SUZ12 antibody (orb1274421) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using SUZ12 antibody (orb1274421) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using SUZ12 antibody (orb1274421) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SUZ12 antibody (orb1274421) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using SUZ12 Polyclonal Antibody (orb1274421) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).
Quick Database Links
Gene Symbol
SUZ12
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
SUZ12 Antibody (orb1274421)
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