Survivin Rabbit Polyclonal Antibody

SKU: orb1294349

Description

Rabbit polyclonal antibody to Survivin

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:2000-1:3000, Immunofluorescence 1:400-1:800, Immunohistochemistry (Paraffin) 1:100-1:300
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Target: Survivin / BIRC5
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. anti-Survivin / BIRC5 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling Survivin / BIRC5 with orb1294349 at 1/150.

Immunohistochemical analysis of paraffin embedded Human Tonsil cancer tissue labeling Survivin / BIRC5 with orb1294349 at 1/150.

Survivin / BIRC5 antibody at 1/2000 dilution. Lysates at 40 µg per lane. This blot was produced using a 15% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294349 overnight at 4°C.

Quick Database Links

Gene Symbol

Survivin / BIRC5