Surfactant protein D Antibody / SFTPD

• Catalog Number: orb2635313
• Category: Antibodies
• Description: Pulmonary surfactant is primarily responsible for lowering the surface tension at the air-liquid interface in the alveoli, a process that is essential for normal respiration. Pulmonary surfactant is a mixture of phospholipids and proteins, including four distinct surfactant-associated proteins (SPs), SP-A, SP-B, SP-C, SP-D. SP-B and SP-C are predominantly hydrophobic proteins that associate with lipids to promote the absorption of surfactant phospholipids and to reduce the surface tension in the alveoli. SP-A and SP-D are large multimeric proteins belonging to the family of calcium-dependent lectins, designated Collectins, which contribute to the innate immune system. Both SP-A and SP-D have been shown to protect against microbial challenge through binding to the lipid components of the bacterial cell wall and facilitating the rapid removal of microbials.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SFTPD/4363
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2c, kappa
• Immunogen: A portion of amino acids 241-336 was used as the immunogen for the Surfactant protein D antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Surfactant protein D antibody is available for research use only.
• UniProt ID: P35247
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the Surfactant protein D antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human lung tissue with Surfactant Protein D antibody (clone SFTPD/4363). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung tissue with Surfactant Protein D antibody (clone SFTPD/4363). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Surfactant protein D antibody (clone SFTPD/4363) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Surfactant protein D antibody (clone SFTPD/4363). These results demonstrate the foremost specificity of the SFTPD/4363 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.