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Item 1 of 6
Item 1 of 6
SUN2 Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb1499408
| Catalog Number | orb1499408 |
|---|---|
| Category | Antibodies |
| Description | SUN2 Recombinant Rabbit Monoclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Recombinant |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | Recombinant protein within human SUN2 aa 500-700. |
| Antibody Type | Recombinant Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, ICC/IF=1:50-100, IF=1:100-500 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 80 kDa |
| Target | SUN2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | FRIGG, KIAA0668, nuclear envelope protein, Protein Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
ICC staining SUN2 (green) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
Western blot analysis of SUN2 on A431 cell lysates with Rabbit anti-SUN2 antibody (orb1499408) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 80 kDa, Observed band size: 75 kDa, Exposure time: 2 minutes, 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (orb1499408) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300000 dilution was used for 1 hour at room temperature.
