SUMO1 Antibody (N-term)

• Catalog Number: orb1936466
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB33652
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 11557 Da
• Target: This SUMO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SUMO1.
• UniProt ID: P63165
• NCBI: NP_001005782.1, NP_003343.1, NP_001005781.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Small ubiquitin-related modifier 1, SUMO-1, GAP-mo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Fluorescent image of A549 cell stained with SUMO1 Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SUMO1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). SUMO1 immunoreactivity is localized to Nuclear Membrane significantly.

SUMO1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SUMO1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

SUMO1 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35 ug/lane). This demonstrates the SUMO1 antibody detected the SUMO1 protein (arrow).

Western blot analysis of lysate from mouse Neuro-2a cell line, using SUMO1 Antibody (N-term). diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary Antibody. Lysate at 35 ug.