SUMO1 Antibody (C-term)

• Catalog Number: orb1937645
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB0632
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 11557 Da
• Target: This SUMO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-86 amino acids from the C-terminal region of human SUMO1.
• UniProt ID: P63165
• NCBI: NP_001005782.1, NP_003343.1, NP_001005781.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Small ubiquitin-related modifier 1, SUMO-1, GAP-mo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels). Untreated (-) and H2O2-treated (+) cells were collected for immunoblot analysis. Top panels: cell lysates probed by western blot (WB) with an anti-ERM Antibody. Center panels: cell lysates immunoprecipitated (IP) with an anti-FLAG antibody followed by WB with SUMO-1 Antibody. Bottom panels: cell lysates immunoprecipitated with an anti-FLAG antibody followed by WB with SUMO-2/3 Antibody. (*) represents immunoprecipitated ERM-like forms recognized by anti-SUMO antibodies.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

SUMO1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

The anti-SUMO1 polyclonal antibody is used in Western blot to detect GST-SUMO1 fusion protein.